This Application Note describes the determination of proteins using the Biuret method. The method is preprogrammed on the corresponding Spectroquant® Prove photometers with firmware version 1.5 …

For Researchers, Scientists, and Drug Development Professionals This guide provides a comprehensive overview of the Biuret test, a widely used colorimetric assay for the detection and quantification of …

Biuret assay is commonly used to determine the total protein in biological samples. Peptides with more than two peptide bonds form a purple colored chelate complex with cupric ions in alkaline medium …

The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer’s law is obeyed to at least 10 g per dl. Several stable biuret reagents are available.

Use clear, colorless and practically neutral liquid samples directly. Filter or centrifuge turbid solutions. Degas samples containing carbon dioxide.

The Biuret test is commonly used in clinical chemistry to detect protein levels in blood and urine, which can provide diagnostic information about a patient’s kidney function and inflammation, among other …

If the solution turns purple it means that protein is present. The method described here uses quantitative Biuret solution which will give a good estimate of protein concentrations in the range 0.1-10 mg/cm3.