Here, we present a cloning strategy for the production of recombinant proteins tagged with a polyhistidine sequence that can be cleaved by the exopeptidase, DAPase. The method can be used with most ...

Colony picking is a very labor-intensive task that is generally performed during cloning protocols, alongside additional upstream and downstream processes. Manual colony picking is both slow and ...

Mammalian cell-based production systems are gaining increasing importance in the manufacture of biotherapeutics. The major advantage of mammalian cell expression platforms over bacteria and yeast is ...

Positional cloning is a genetic mapping technique used to pinpoint the location of specific traits of interest, such as disease-causing genes or mutations, within the genome. Very simply, this ...